Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Pfanstiehl Inc Tris Hydrochloride (Tris HCl) High Purity, Low Endotoxin, Low Metals (T-154) 5kg GMP CAS: 1185-53-1 Injectable Excipient Grade
Pfanstiehl Tris HCL, High Purity, Low Endotoxin, Low Metals, is a highly purified buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris HCl meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. cGMP , pharmaceutical grade .
Teknova DNA Suspension Buffer, pH 8.0, 10L, DNase/RNase Tested, PCR Grade, Sterile. 2 Pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Medchemexpress LLC 1H-1,4,7-triazonine-1,4,7-triacetic acid, α1-(2-carboxyethyl)hexahydro-, tris(tert-butyl) ester | 1190101-34-8 | MFCD29083820 | 99.8% | 543.69 g/mol | C27H49N3O8 | 50 MG
NODAGA-tris(t-Bu ester) is a tert-butyl-protected bifunctional chelator precursor used for covalent attachment to biomolecules. It is designed to conjugate to peptides, proteins, or antibodies; after deprotection the chelator can be radiolabeled for PET imaging studies. The reagent is supplied as a high-purity research chemical suitable for labeling workflows and analytical characterization.
- Tert-butyl protected chelator precursor suitable for conjugation to peptides and antibodies.
- Compatible with deprotection and subsequent radiolabeling for PET imaging.
- High purity as measured by HPLC, appropriate for research use.
- Soluble in DMSO for formulation and conjugation workflows.
- Molecular weight 543.69 g/mol; chemical formula C27H49N3O8.
Bioworld TAE Buffer (50X), 4 L
Product DescriptionTAE (Tris-Acetate-EDTA) buffer liquid concentrate has a 50X concentration and is filtered through a 0.22 µm membrane. It has a pH of 8.5 ± 0.1. A concentration of 1X contains 0.04M Tris, 0.02M acetate, and 0.001M EDTA. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Apexbio Technology LLC PBS Liposomes(Synonyms: Phosphate Buffered Saline Liposomes, Empty Liposomes, Blank Liposomes), 10mL.
PBS Liposomes consist of phosphate-buffered saline (PBS) encapsulated within lipid bilayers serving primarily as control reagents in macrophage depletion studies involving clodronate liposomes Unlike clodronate-loaded liposomes PBS liposomes lack active agents targeting macrophages and thus do not induce macrophage apoptosis They function to discriminate between the biological effects mediated by liposomal delivery systems versus those specifically attributable to encapsulated clodronate PBS Liposomes are commonly employed in comparative experimental designs to assess specific macrophage depletion outcomes
Biotium Ready-to-Use Tyramide Amplification Buffer, 1X
Ready-to-use tyramide amplification buffer can be used for tyramide signal amplification with CF Dye Tyramides or other tyramides without the need to add hydrogen peroxide.
PLANT CELL TECHNOLOGY 1X Phosphate Buffered Saline Solution, pH 7.4, without Calcium and Magnesium, Buffer Solution, Mod. 6, Clear Liquid, Sterile, 500mL bottle.
1X PBS Solution Mod. 6, pH 7.4, is a sterile buffer used in mammalian cell culture to maintain physiological pH during washing, dilution, or reagent preparation. Packaged in a clear 500mL bottle. Ready-to-use and stable under recommended conditions. Ideal for research labs, tissue culture, and molecular biology workflows.
Mitegen Tris/ HCl, 1M, pH8.5, 100 ml, CAS # 77-86-1
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Sigma Aldrich Fine Chemicals Biosciences Trizma base solution | 77-86-1 | MFCD00004679 | 100 mL
Trizma base solution | Mol Wt: 121.135 | 77-86-1 | MFCD00004679 | 100 mL
Electron Microscopy Sciences Hank's Balanced Salts Formula II 500 ML
All of our Hanks' Balanced Salt Solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All solutions are prepared in 1X strength.