Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | 1185-53-1 | MFCD00012590 | 100G
Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 157.6 | 1185-53-1 | MFCD00012590 | 100G
Sigma Aldrich Fine Chemicals Biosciences Tris hydrochloride | 1185-53-1 | MFCD00012590
Tris hydrochloridePurity: | MW: 157.6 | 1185-53-1 | MFCD00012590
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 5kg
Ethylenediaminetetraacetic acid | Purity: ≥98.5%, powder | MW:292.24 | 60-00-4 | MFCD00003541 | 5kg
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | 60-00-4 | MFCD00003541 | 250G
Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | Purity: >=99.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 250G
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid anhydrous, crystalline, BioReagent, suitable for cell culture | 60-00-4 | MFCD00003541 | 500g
Ethylenediaminetetraacetic acid anhydrous, crystalline, BioReagent, suitable for cell culture | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500g
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid purified grade, >=98.5%, powder | 60-00-4 | MFCD00003541 | 500G
Ethylenediaminetetraacetic acid purified grade, >=98.5%, powder | Purity: >=98.5% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500G
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | 60-00-4 | MFCD00003541 | 500G
Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | Purity: 99.4-100.6% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500G
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid >=98.0% (KT) | 60-00-4 | MFCD00003541 | 50G
Ethylenediaminetetraacetic acid >=98.0% (KT) | Purity: >=98.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 50G
Electron Microscopy Sciences Tris Buffered Saline, pH 7.4 250 ML
C₄H₁₁NO₃ F.W. 121.14
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged.
pH: 7.4
Electron Microscopy Sciences Tris HCL Buffer, pH range 7.2-9.0 250 ML
Please specify pH when ordering.
pH: 7.2-9.0
Sigma Aldrich Fine Chemicals Biosciences MOBS >=99% (titration) | 115724-21-5 | MFCD00674916 | 100G
MOBS >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 223.29 | 115724-21-5 | MFCD00674916 | 100G
Sigma Aldrich Fine Chemicals Biosciences BICINE BioXtra 99 titratio1KG
BICINE BioXtra 99 titratio1KG
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Sigma Aldrich Fine Chemicals Biosciences HEPES potassium salt 99.5250G
HEPES potassium salt 99.5250G